RAPD Analysis of the Genetic Diversity Among Accessions of Micropropagation Bananas from Indonesia

RAPD Analysis of the Genetic Diversity Among Accessions of Micropropagation Bananas from Indonesia

Susilo, Handayani, Satya Darmayani, Muh. Shofi, Anita Restu Puji Raharjeng

Molecular Characterization of banana (Musa spp) is very important for the purpose of conservation and improvement of plants. This research was conducted to investigate phylogenetic of Musa spp cultivar namely Cavendish, Barangan, Raja Buluh and Kepok. RAPD analysis was performed on four accessions of Musa spp, originating from West Java, Indonesia. The samples used were taken from the plant result of micropropagation at Tissue Culture laboratory Lebak Bulus, Jakarta, Indonesia. The result of PCR-RAPD visualization produces bands whose size ranges from 200-1500 bp. The primers used were able to amplify four samples clearly. The DNA amplification results show that the four primers can produce 62 polymorphic and monomorphic bands. A total of 30 polymorphic bands is capable of producing on PCR-RAPD process with 48.29%. Primer OPB 3 produces the most polymorphic bands of 10 bands whereas OPB 4 produces only four polymorphic bands. Primer OPB 3 is capable of producing the highest percentage of polymorphic loci that is 55.56%. Dendogram results show that at the coefficient of 0.68 separation occurs in the Raja Buluh with other samples. While in the coefficient of 0.70, the varieties of Kepok shows the separation of Raja Buluh and Cavendish. At the coefficient of 0.90, the Barangan and Cavendish varieties are in the same branching which tools that the two have close phylogenetic. RAPD sequence analysis has provided more information on the inter-species phylogenetic status of these four cultivars of Musa spp.

2018

Journal of Physics: Conference Series, Volume 1114, 2018
doi :10.1088/1742-6596/1114/1/012137
https://iopscience.iop.org/article/10.1088/1742-6596/1114/1/012137